Pregnenolone

Pregnenolone (3β-hydroxypregn-5-en-20-one), also known as P5, is an endogenous steroid hormone. It is the precursor of the progestogens, mineralocorticoids, glucocorticoids, androgens, and estrogens, as well as the neuroactive steroids. In addition, pregnenolone is biologically active in its own right, acting as a neurosteroid.

Chemistry:

Like other steroids, pregnenolone consists of four interconnected cyclic hydrocarbons. It contains ketone and hydroxyl functional groups, two methyl branches, and a double bond at C5, in the B cyclic hydrocarbon ring. Like many steroid hormones, it is hydrophobic. The sulfated derivative, pregnenolone sulfate, is water-soluble.

Biology

Biosynthesis:

Pregnenolone is synthesized from cholesterol. This conversion involves hydroxylation at the side-chain at C20 and C22 positions, with cleavage of the side-chain. The enzyme performing this task is cytochrome P450scc, located in the mitochondria, and controlled by anterior pituitary tropic hormones, such as ACTH, FSH, LH.

To assay conversion of cholesterol to pregnenolone, radiolabelled cholesterol has been used. Pregnenolone product can be separated from cholesterol substrate using Sephadex LH-20 minicolumns.

Product Description

English name: Pregnenolone

CAS: 145-13-1

EINECS Number: 205-647-4

Formula: C21H32O2

MW: 316.4776

Density: 1.09g / cm3

Melting point: 190-193 ℃

Boiling point: 443.3 ° C at 760 mmHg

Flash Point: 188.9 ° C

Vapor Pressure: 1.02E-09mmHg at 25 ° C

Properties:

Melting point 190-193 ° C

Specific rotation 28.5 ° (c = 1, EtOH)

Uses:Used as pharmaceutical intermediates progesterone

Assay: 99%

Appearance: White crystalline powder

specification of Pregnenolone:

Item	Specification
Appearance	White or almost white crystalline powder or colorless crystals
Solubility	Practically insoluble in water, soluble in ethanol, sparingly soluble in acetone and fatty oil
Identification	IR: Complies with the reference spectrum
	TLC: Complies
Specific optical rotation	+186° to +194°(Calculated on the dry basis)
Related substances	Total impurities:≤0.8%
	Impurity A: ≤0.5%
	Impurity B: ≤0.5%
	Impurity C: ≤0.5%
	Impurity D: ≤0.5%
	Impurity E: ≤0.5%
	Impurity F: ≤0.5%
	Impurity G: ≤0.5%
	(Individual unidentified impurities): ≤0.10%
Loss on drying	≤0.5%
Assay(UV)	97.0% to 103.0%(Calculated on the dry basis)

packing&storage:

package:25kg/drum,double plastic-bag inside,aluminum foil bag or fiber drum outside.

storage:stored in a clean,cool, dry area;keep away frommoisture and stong,direct light/heat.

shelf life: two years when properly stored

application of Pregnenolone:

1. Used in the synthesis of drug intermediate 

2. Used as intermediate for the synthesis of progesterone, antiflamison;

3. With the same function of corticosteroids anti-inflammatory.

my contact infomation is:

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Notice: This product is only suitable for laboratory use, or the use of animals. It can not be used directly on the human body